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actin filaments  (Cytoskeleton Inc)


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    Structured Review

    Cytoskeleton Inc actin filaments
    Actin Filaments, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/actin+stain+555+phalloidin/Acti-stain+555+phalloidin/bio_rxiv__64898__2026__05__12__724633-268-0-8
    Average 96 stars, based on 364 article reviews
    actin filaments - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Methionine oxidation of actin cytoskeleton attenuates traumatic memory retention via reactivating dendritic spine morphogenesis.
    Article Snippet: Subsequently, the slices were labeled with Actin-stainTM 555 phalloidin (1:200, Cytoskeleton lnc., Denver, USA) for 1 h at room temperature in the dark, followed by 3 times washes with PBS.

    Article Title: Methionine oxidation of actin cytoskeleton attenuates traumatic memory retention via reactivating dendritic spine morphogenesis
    Article Snippet: Subsequently, the slices were labeled with Actin-stainTM 555 phalloidin (1:200, Cytoskeleton lnc., Denver, USA) for 1 h at room temperature in the dark, followed by 3 times washes with PBS.

    Article Title: MLCK/MLCP regulates mammalian axon regeneration via the redistribution of the growth cone F-actin
    Article Snippet: Actin-stain 555 phalloidin (Cat # PHDH1) was purchased from Cytoskeleton, Inc. siRNAs designed to target mouse MLCK (siMLCK) (5′-GGCAAATACACCTGTGAAG-3′, 5′-TGGTCAAAGAAGGGCAGAT-3′, 5′-AGCCAAAGGGAGTCAACAT-3′, and 5′-TCACGACGGGAATGAGATT-3′) and mouse MYPT1 (siMYPT1) (5′-CTGTGGATATCTCGATATTGC-3′) were obtained from GenePharma (Shanghai, China).

    Staining:

    Article Title: PPARγ Antagonists Exhibit Antitumor Effects by Regulating Ferroptosis and Disulfidptosis
    Article Snippet: .. After fixation, the cells were permeabilized with a permeabilization buffer (0.5% Triton X-100 in PBS) for 5 min. Actin filaments were stained with 100 nM Actin-stain 555 phalloidin (Cytoskeleton, Denver, CO, USA) for 30 min. .. The slides were then mounted with a DAPI-containing mounting medium (VECTASHIELD, Vector Labs, Burlingame, CA, USA) and examined under a fluorescence microscope.

    Article Title: Role of TFEB in regulation of the podocyte actin cytoskeleton.
    Article Snippet: Then, the podocytes were incubated with rabbit anti-TFEB (1:200, Bethyl Laboratories, Texas, USA) and mouse anti-Synaptopodin (1:100, Santa Cruz Biotechnology, Texas, USA) overnight at 4 ◦C. .. After three washing with PBS, podocytes were incubated with goat anti-rabbit Alexa Fluor 488 (1:500, Cell Signaling Technology, MA, USA) and goat anti-mouse Alexa Fluor 555 (1:500, Cell Signaling Technology, MA, USA) or the actin stain 555 phalloidin (1:140, Cytoskeleton, Denver, USA) for 1 h at room temperature avoiding light. .. After washing three times, the slides were mounted with anti-fade mounting medium and 4,6-diamino-2-phenylindole (DAPI) (Beyotime Institute of Biotechnology, China).

    Article Title: A polysaccharide gel made of gellan gum improves oocyte maturation and embryonic development in pigs.
    Article Snippet: .. The oocytes were then incubated in a permeabilization solution (0.25% Triton X/PBS) for 30 min, followed by F-actin staining and staining with Actin-stain 555 phalloidin (Cytoskeleton, Denver, CO, USA) for 30 min. .. They were then mounted on a glass slide and observed under a Leica DMI 6000 B microscope using the LAS AF software (Leica).

    Article Title: A polysaccharide gel made of gellan gum improves oocyte maturation and embryonic development in pigs
    Article Snippet: .. The oocytes were then incubated in a permeabilization solution (0.25% Triton X/PBS) for 30 min, followed by F-actin staining and staining with Actin-stain 555 phalloidin (Cytoskeleton, Denver, CO, USA) for 30 min. .. They were then mounted on a glass slide and observed under a Leica DMI 6000 B microscope using the LAS AF software (Leica).

    Incubation:

    Article Title: Capturing nematic order on tissue surfaces of arbitrary geometry
    Article Snippet: HUVECs were cultured for 3 days as 3D micro-vessel using specialized extracellular matrix consisting of 1.2 mg/ml polyisocyanopeptide (PIC) hybridized with 2 mg/ml type 1 atelo bovine collagen (Advanced Biomatrix, 5010) . .. The micro-vessel was fixed and incubated overnight with Actin-Stain 555 Phalloidin (1:400, PHDH1-A, Cytoskeleton) to label F-actin. ..

    Article Title: Role of TFEB in regulation of the podocyte actin cytoskeleton.
    Article Snippet: Then, the podocytes were incubated with rabbit anti-TFEB (1:200, Bethyl Laboratories, Texas, USA) and mouse anti-Synaptopodin (1:100, Santa Cruz Biotechnology, Texas, USA) overnight at 4 ◦C. .. After three washing with PBS, podocytes were incubated with goat anti-rabbit Alexa Fluor 488 (1:500, Cell Signaling Technology, MA, USA) and goat anti-mouse Alexa Fluor 555 (1:500, Cell Signaling Technology, MA, USA) or the actin stain 555 phalloidin (1:140, Cytoskeleton, Denver, USA) for 1 h at room temperature avoiding light. .. After washing three times, the slides were mounted with anti-fade mounting medium and 4,6-diamino-2-phenylindole (DAPI) (Beyotime Institute of Biotechnology, China).

    Article Title: A polysaccharide gel made of gellan gum improves oocyte maturation and embryonic development in pigs.
    Article Snippet: .. The oocytes were then incubated in a permeabilization solution (0.25% Triton X/PBS) for 30 min, followed by F-actin staining and staining with Actin-stain 555 phalloidin (Cytoskeleton, Denver, CO, USA) for 30 min. .. They were then mounted on a glass slide and observed under a Leica DMI 6000 B microscope using the LAS AF software (Leica).

    Article Title: A polysaccharide gel made of gellan gum improves oocyte maturation and embryonic development in pigs
    Article Snippet: .. The oocytes were then incubated in a permeabilization solution (0.25% Triton X/PBS) for 30 min, followed by F-actin staining and staining with Actin-stain 555 phalloidin (Cytoskeleton, Denver, CO, USA) for 30 min. .. They were then mounted on a glass slide and observed under a Leica DMI 6000 B microscope using the LAS AF software (Leica).



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    Image Search Results


    CSL305 inhibition of C3b deposition on endothelial cells in vitro. Microfluidic chips were cultured with human lung microvascular endothelial cells over two days under flow conditions. Cells were opsonized with an anti-CD105 antibody or left un-opsonized and perfused with normal human serum to mimic inflammatory conditions. CSL305 at 25, 50, 100, and 500 nM was added to opsonized cells in separate fluidic channels, as was the WT anti-C2 mAb (500 nM) and CSL040 (100 nM) as controls. Cells were then fixed and stained with F-actin (Acti-Stain™ 555), C3b/c (conjugated to FITC), or DAPI. ( A ) Representative images from each condition. Results from N = 4 experiments are shown graphically in ( B ) violin plots as a measure of C3b fluorescence intensity. A total of 3–4 images (regions of interest) were analyzed per condition per experiment, with some conditions limited to 3 experiments due to channel number limitations, and mean grey values of C3b intensity are represented as single dots (9–16 data points per condition). Statistical analyses were performed using a Kruskal–Wallis test with multiple comparisons; n.s., not significant; ** p < 0.01; *** p < 0.005; **** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: CSL305: A Dual Functional Therapeutic Antibody Targeting Complement C2 and FcRn

    doi: 10.3390/ijms27052383

    Figure Lengend Snippet: CSL305 inhibition of C3b deposition on endothelial cells in vitro. Microfluidic chips were cultured with human lung microvascular endothelial cells over two days under flow conditions. Cells were opsonized with an anti-CD105 antibody or left un-opsonized and perfused with normal human serum to mimic inflammatory conditions. CSL305 at 25, 50, 100, and 500 nM was added to opsonized cells in separate fluidic channels, as was the WT anti-C2 mAb (500 nM) and CSL040 (100 nM) as controls. Cells were then fixed and stained with F-actin (Acti-Stain™ 555), C3b/c (conjugated to FITC), or DAPI. ( A ) Representative images from each condition. Results from N = 4 experiments are shown graphically in ( B ) violin plots as a measure of C3b fluorescence intensity. A total of 3–4 images (regions of interest) were analyzed per condition per experiment, with some conditions limited to 3 experiments due to channel number limitations, and mean grey values of C3b intensity are represented as single dots (9–16 data points per condition). Statistical analyses were performed using a Kruskal–Wallis test with multiple comparisons; n.s., not significant; ** p < 0.01; *** p < 0.005; **** p < 0.001.

    Article Snippet: A fluorescein isothiocyanate (FITC)-coupled antibody against C3b/c (Rabbit Anti-Human C3c-FITC, 1:100, Agilent Technologies, Lausanne, Switzerland), F-actin (Acti-Stain 555 Phalloidin, 1:200, Cytoskeleton, Inc., Denver, CO, USA), and DAPI stain (1:1000, Sigma Aldrich, Saint Louis, MO, USA) were diluted in staining solution (PBS, 1% bovine serum albumin, 0.05% Tween, Sigma Aldrich, Saint Louis, MO, USA) and incubated in the microchannels for 3 h at RT or overnight at 4 °C.

    Techniques: Inhibition, In Vitro, Cell Culture, Staining, Fluorescence